Type II NKT cells facilitate Alum-sensing and humoral immunity

Journal of Leukocyte Biology

Gillian A. Lang¹  Hemangi B. Shah¹  Mark L. Lang¹  Pragya Rampuria¹  T. Scott Devera¹ 

[1] Department of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USADepartment of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USADepartment of Microbiology and Immunology, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

关键词: adjuvant;    B cell;    T cell;    cytokine;    plasma cell;   

DOI  :  10.1189/jlb.0412177

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Alum-based adjuvants facilitate vaccine-driven humoral immunity, but their mechanism of action remains poorly understood. Herein, we report that lack of type II NKT cells is associated with intact, mature B cells but dampened humoral immunity following immunization with Alum-adsorbed T-dependent antigen. Type II NKT cells facilitated production of IL-4, IL-5, IL-10, IL-13, and antibody by LN and splenocyte cultures following Alum/antigen administration in vivo and antigen restimulation in vitro. Addition of IL-4 and IL-5 to type II NKT-deficient cultures restored in vitro antibody production. Intracellular staining revealed that Alum-primed type II NKT cells coordinated IL-4 secretion by T cells. Alum did not significantly affect CD1d expression in vivo, but addition of CD1d-blocking mAb diminished cytokine production and in vitro antibody production. Type II NKT cells therefore function as part of the Alum-sensing apparatus and in a CD1d-dependent manner, facilitate TH2-driven humoral immunity. This may have important consequences for understanding the mechanism of action of Alum-containing vaccines.

【 授权许可】

Unknown