Type II NKT cells facilitate Alum‐sensing and humoral immunity

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: adjuvant;    B cell;    T cell;    cytokine;    plasma cell;   

DOI  :  10.1189/jlb.0412177

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Alum‐basedadjuvantsfacilitatevaccine‐drivenhumoralimmunity,buttheirmechanismofactionremainspoorlyunderstood.Herein,wereportthatlackoftypeIINKTcellsisassociatedwithintact,matureBcellsbutdampenedhumoralimmunityfollowingimmunizationwithAlum‐adsorbedT‐dependentantigen.TypeIINKTcellsfacilitatedproductionofIL‐4,IL‐5,IL‐10,IL‐13,andantibodybyLNandsplenocyteculturesfollowingAlum/antigenadministrationinvivoandantigenrestimulationinvitro.AdditionofIL‐4andIL‐5totypeIINKT‐deficientculturesrestoredinvitroantibodyproduction.IntracellularstainingrevealedthatAlum‐primedtypeIINKTcellscoordinatedIL‐4secretionbyTcells.AlumdidnotsignificantlyaffectCD1dexpressioninvivo,butadditionofCD1d‐blockingmAbdiminishedcytokineproductionandinvitroantibodyproduction.TypeIINKTcellsthereforefunctionaspartoftheAlum‐sensingapparatusandinaCD1d‐dependentmanner,facilitateTH2‐drivenhumoralimmunity.ThismayhaveimportantconsequencesforunderstandingthemechanismofactionofAlum‐containingvaccines...

【 授权许可】

CC BY