Activation of NK cell cytotoxicity by the natural compound 2,3-butanediol

Journal of Leukocyte Biology

Chih-Jung Chang²  Hsin-Chih Lai⁷  Chia-Chen Lu#⁵  Ya-Jing Hsu³  Chun-Hung Yang⁴  Yu-Huan Tsai³  Chang-Chieh Chen³  Tsung-Teng Huang⁷  Ying-Huang Tsai and¹  David M. Ojcius –⁶  Chuan-Sheng Lin³ 

[1] Department of Pulmonary and Critical Care Chang Gung Memorial Hospital, Chia Yi Branch, Taiwan, Republic of China; and #Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of China;Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taiwan, Republic of China; #Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of ChinaDepartment of Medical Biotechnology and Laboratory Science, #Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of China Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taiwan, Republic of China;;Department of Medical Biotechnology and Laboratory Science, Division of Applied Toxicology, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Taiwan, Republic of China; #Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of China#Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of China Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taiwan, Republic of China;; Center for Pathogenic Bacteria and Center for Molecular and Clinical Immunology, –Health Sciences Research Institute and School of Natural Sciences, University of California Merced, Merced, California, USA; #Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of ChinaDepartment of Medical Biotechnology and Laboratory Science,  Center for Pathogenic Bacteria and Center for Molecular and Clinical Immunology, #Department of Respiratory Therapy, College of Medicine, Fu Jen Catholic University, Taiwan, Republic of China Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taiwan, Republic of China;

关键词: perforin;    NKG2D;    NCR;    resveratrol;   

DOI  :  10.1189/jlb.0112024

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The natural compound 2,3-BTD has diverse physiological effects in a range of organisms, including acting as a detoxifying product of liver alcohol metabolism in humans and ameliorating endotoxin-induced acute lung injury in rats. In this study, we reveal that 2,3-BTD enhances NK cell cytotoxic activity in human pNK cells and NK92 cells. Treatment of NK cells with 2,3-BTD increased perforin expression in a dose-dependent manner. This was accompanied by elevated JNK and ERK1/2 MAPK activities and enhanced expression of NKG2D/NCRs, upstream signaling molecules of the MAPK pathways. The 2,3-BTD effect was inhibited by pretreatment with inhibitors of JNK (SP) or ERK1/2 (PD) or by depleting NKG2D/NCRs or JNK1 or ERK2 with siRNA. These results indicate that 2,3-BTD activates NK cell cytotoxicity by NKG2D/NCR pathways and represent the first report of the 2,3-BTD effect on activation of innate immunity cells.

【 授权许可】

Unknown