The mouse interleukin (Il)33 gene is expressed in a cell type- and stimulus-dependent manner from two alternative promoters

Journal of Leukocyte Biology

Cem Gabay¹  Nicolas Calo¹  Solenne Vigne¹  Gaby Palmer¹  Dominique Talabot-Ayer¹  Céline Lamacchia¹ 

[1] Division of Rheumatology, University Hospital, and Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland Division of Rheumatology, University Hospital, and Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland Division of Rheumatology, University Hospital, and Department of Pathology and Immunology, University of Geneva School of Medicine, Geneva, Switzerland

关键词: dendritic cells;    inflammation;    macrophages;    myeloid cells;    TLR ligands;   

DOI  :  10.1189/jlb.0811425

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

GenBank entries for mouse Il33 reveal the existence of two transcripts, Il33a and Il33b, with different 5′UTRs but coding for the same protein. We investigated expression of these transcripts in different mouse organs and cell types in basal and inflammatory conditions. Il33a and Il33b mRNAs start with different noncoding first exons, transcribed from different promoter regions, which both contain a consensus TATA-like sequence. Constitutive Il33a mRNA expression was detected in mouse stomach, lung, spleen, and brain, whereas basal Il33b mRNA expression was observed only in the stomach. Expression of both transcripts increased after systemic LPS administration. In vitro, we observed high constitutive expression of Il33 transcripts in MEFs. Constitutive Il33a mRNA expression was observed also in BMDCs, where it was preferentially increased in response to poly(I:C), whereas LPS increased levels of Il33a and Il33b mRNA. In contrast, BMMs and Raw 264.7 cells did not express Il33 mRNA constitutively, and LPS stimulation selectively induced expression of Il33b mRNA in these cells. Our data indicate that the Il33 gene is expressed from two alternative promoters in the mouse and that the relative expression of Il33a and Il33b transcripts is cell type- and stimulus-dependent.

【 授权许可】

Unknown