The mouse interleukin (Il)33 gene is expressed in a cell type‐ and stimulus‐dependent manner from two alternative promoters

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: dendritic cells;    inflammation;    macrophages;    myeloid cells;    TLR ligands;   

DOI  :  10.1189/jlb.0811425

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

GenBankentriesformouseIl33revealtheexistenceoftwotranscripts,Il33aandIl33b,withdifferent5′UTRsbutcodingforthesameprotein.Weinvestigatedexpressionofthesetranscriptsindifferentmouseorgansandcelltypesinbasalandinflammatoryconditions.Il33aandIl33bmRNAsstartwithdifferentnoncodingfirstexons,transcribedfromdifferentpromoterregions,whichbothcontainaconsensusTATA‐likesequence.ConstitutiveIl33amRNAexpressionwasdetectedinmousestomach,lung,spleen,andbrain,whereasbasalIl33bmRNAexpressionwasobservedonlyinthestomach.ExpressionofbothtranscriptsincreasedaftersystemicLPSadministration.Invitro,weobservedhighconstitutiveexpressionofIl33transcriptsinMEFs.ConstitutiveIl33amRNAexpressionwasobservedalsoinBMDCs,whereitwaspreferentiallyincreasedinresponsetopoly(I:C),whereasLPSincreasedlevelsofIl33aandIl33bmRNA.Incontrast,BMMsandRaw264.7cellsdidnotexpressIl33mRNAconstitutively,andLPSstimulationselectivelyinducedexpressionofIl33bmRNAinthesecells.OurdataindicatethattheIl33geneisexpressedfromtwoalternativepromotersinthemouseandthattherelativeexpressionofIl33aandIl33btranscriptsiscelltype‐andstimulus‐dependent...

【 授权许可】

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