Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
The mouse interleukin (Il)33 gene is expressed in a cell type‐ and stimulus‐dependent manner from two alternative promoters | |
关键词: dendritic cells; inflammation; macrophages; myeloid cells; TLR ligands; | |
DOI : 10.1189/jlb.0811425 | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
GenBankentriesformouseIl33revealtheexistenceoftwotranscripts,Il33aandIl33b,withdifferent5′UTRsbutcodingforthesameprotein.Weinvestigatedexpressionofthesetranscriptsindifferentmouseorgansandcelltypesinbasalandinflammatoryconditions.Il33aandIl33bmRNAsstartwithdifferentnoncodingfirstexons,transcribedfromdifferentpromoterregions,whichbothcontainaconsensusTATA‐likesequence.ConstitutiveIl33amRNAexpressionwasdetectedinmousestomach,lung,spleen,andbrain,whereasbasalIl33bmRNAexpressionwasobservedonlyinthestomach.ExpressionofbothtranscriptsincreasedaftersystemicLPSadministration.Invitro,weobservedhighconstitutiveexpressionofIl33transcriptsinMEFs.ConstitutiveIl33amRNAexpressionwasobservedalsoinBMDCs,whereitwaspreferentiallyincreasedinresponsetopoly(I:C),whereasLPSincreasedlevelsofIl33aandIl33bmRNA.Incontrast,BMMsandRaw264.7cellsdidnotexpressIl33mRNAconstitutively,andLPSstimulationselectivelyinducedexpressionofIl33bmRNAinthesecells.OurdataindicatethattheIl33geneisexpressedfromtwoalternativepromotersinthemouseandthattherelativeexpressionofIl33aandIl33btranscriptsiscelltype‐andstimulus‐dependent...
【 授权许可】
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