Technical Advance: Immunophenotypical characterization of human neutrophil differentiation

Journal of Leukocyte Biology

Niels Borregaard, and²  Helena Mora-Jensen²  Bo Porse¹  Johan Jendholm¹  Anna Fossum³  Kim Theilgaard-Mönch, ,⁴ 

[1]  The Laboratory for Gene Therapy Research, and Biotech and Research Innovation Centre, University of Copenhagen, Copenhagen, Denmark;The Granulocyte Research Laboratory, Department of Hematology, Biotech and Research Innovation Centre, University of Copenhagen, Copenhagen, Denmark;Biotech and Research Innovation Centre, University of Copenhagen, Copenhagen, Denmark;;The Granulocyte Research Laboratory, Department of Hematology,  The Laboratory for Gene Therapy Research, and Biotech and Research Innovation Centre, University of Copenhagen, Copenhagen, Denmark; and Department of Hematology, SkÃ¥ne University Hospital, University of Lund, Lund, Sweden

关键词: granulocytes;    myeloid differentiation;    differentiation pathway;    flow cytometry;    human bone marrow;   

DOI  :  10.1189/jlb.0311123

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

【 授权许可】

Unknown