期刊论文详细信息
| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Technical Advance: Immunophenotypical characterization of human neutrophil differentiation | |
| 关键词: granulocytes; myeloid differentiation; differentiation pathway; flow cytometry; human bone marrow; | |
| DOI : 10.1189/jlb.0311123 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
Thecurrentstudyreportsaflowcytometry‐basedprotocolfortheprospectivepurificationofhumanBMpopulationsrepresentingsixsuccessivestagesofterminalneutrophildifferentiation,includingearlypromyelocytesandlatepromyelocytes,myelocytes,metamyelocytes,bandcells,andPMNneutrophilicgranulocytes.Validationexperimentsrevealedahighpurityofeachbonemarrowpopulationandbiologicalmeaningfulexpressionprofilesformarkergenesofneutrophildifferentiationatahithertounprecedentedresolution.Hence,thepresentprotocolshouldbeusefulforstudyingneutrophildifferentiationinvivointhehumansettingandconstitutesanimportantalternativetomodelsthatarebasedoninvitrodifferentiationofmyeloidcelllinesandHPCs...
【 授权许可】
CC BY
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201904048756873ZK.pdf | 705KB |  download |
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