Journal of Leukocyte Biology | |
Cofilin regulator 14-3-3ζ is an evolutionarily conserved protein required for phagocytosis and microbial resistance | |
Milka Vuoksio3  Leena-Maija Vanha-aho3  Sinikka Eskelinen2  Christine Kocks–7  Dan Hultmark1  Mika Rämet 4  Anni Kleino1  Mari Vähä-Mäkilä5  Mataleena Parikka and3  Johanna Ulvila6  Mikko Hallman5  | |
[1] Institute of Biomedical Technology and Department of Molecular Biology, UmeÃ¥ University, UmeÃ¥, Sweden;Pathology, Biocenter Oulu, University of Oulu, Finland; Institute of Biomedical Technology and Pathology, Biocenter Oulu, University of Oulu, Finland; Institute of Biomedical Technology and Department of Pediatrics, University of Tampere, Finland;Departments of Pediatrics and Pathology, Biocenter Oulu, University of Oulu, Finland;Departments of Pediatrics and Institute of Biomedical Technology and Pathology, Biocenter Oulu, University of Oulu, Finland;–Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Pathology, Biocenter Oulu, University of Oulu, Finland; | |
关键词: Drosophila; zebrafish; plasmatocyte; macrophage; immunity; | |
DOI : 10.1189/jlb.0410195 | |
学科分类:生理学 | |
来源: Federation of American Societies for Experimental Biology | |
【 摘 要 】
Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912010182949ZK.pdf | 44KB | download |