Cofilin regulator 14-3-3ζ is an evolutionarily conserved protein required for phagocytosis and microbial resistance

Journal of Leukocyte Biology

Milka Vuoksio³  Leena-Maija Vanha-aho³  Sinikka Eskelinen²  Christine Kocks–⁷  Dan Hultmark¹  Mika Rämet ⁴  Anni Kleino¹  Mari Vähä-Mäkilä⁵  Mataleena Parikka  and³  Johanna Ulvila⁶  Mikko Hallman⁵ 

[1]  Institute of Biomedical Technology and Department of Molecular Biology, UmeÃ¥ University, UmeÃ¥, Sweden;Pathology, Biocenter Oulu, University of Oulu, Finland; Institute of Biomedical Technology and Pathology, Biocenter Oulu, University of Oulu, Finland; Institute of Biomedical Technology and Department of Pediatrics, University of Tampere, Finland;Departments of Pediatrics and Pathology, Biocenter Oulu, University of Oulu, Finland;Departments of Pediatrics and  Institute of Biomedical Technology and Pathology, Biocenter Oulu, University of Oulu, Finland;–Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA Pathology, Biocenter Oulu, University of Oulu, Finland;

关键词: Drosophila;    zebrafish;    plasmatocyte;    macrophage;    immunity;   

DOI  :  10.1189/jlb.0410195

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Phagocytosis is an ancient cellular process that plays an important role in host defense. In Drosophila melanogaster phagocytic, macrophage-like hemocytes recognize and ingest microbes. We performed an RNAi-based in vitro screen in the Drosophila hemocyte cell line S2 and identified Abi, cpa, cofilin regulator 14-3-3ζ, tlk, CG2765, and CG15609 as mediators of bacterial phagocytosis. Of these identified genes, 14-3-3ζ had an evolutionarily conserved role in phagocytosis: bacterial phagocytosis was compromised when 14-3-3ζ was targeted with RNAi in primary Drosophila hemocytes and when the orthologous genes Ywhab and Ywhaz were silenced in zebrafish and mouse RAW 264.7 cells, respectively. In Drosophila and zebrafish infection models, 14-3-3ζ was required for resistance against Staphylococcus aureus. We conclude that 14-3-3ζ is essential for phagocytosis and microbial resistance in insects and vertebrates.

【 授权许可】

Unknown