gcm2 Promotes Glial Cell Differentiation and Is Required with glial cells missing for Macrophage Development in Drosophila

Developmental Biology

Teresa B Alfonso¹  Bradley W Jones¹ 

[1] Molecular Neurobiology Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY, 10016

关键词: glial cells missing;    gcm;    gcm2;    glia;    hemocyte;    plasmatocyte;    macrophage;    blood cells;    Drosophila;   

DOI  :  10.1006/dbio.2002.0740

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

glialcellsmissing(gcm)istheprimaryregulatorofglialcellfateinDrosophila.Inaddition,gcmhasaroleinthedifferentiationoftheplasmatocyte/macrophagelineageofhemocytes.Sincemutationofgcmcausesonlyadecreaseinplasmatocytenumberswithoutchangingtheirabilitytoconvertintomacrophages,gcmcannotbethesoledeterminantofplasmatocyte/macrophagedifferentiation.Wehavecharacterizedagcmhomolog,gcm2.gcm2isexpressedatlowlevelsinglialcellsandhemocyteprecursors.Weshowthatgcm2hasredundantfunctionswithgcmandhasaminorrolepromotingglialcelldifferentiation.Moresignificant,likegcm,mutationofgcm2leadstoreducedplasmatocytenumbers.Adeletionremovingbothgeneshasallowedustoclarifytheroleoftheseredundantgenesinplasmatocytedevelopment.Animalsdeficientforbothgcmandgcm2failtoexpressthemacrophagereceptorCroquemort.Plasmatocytesarereducedinnumber,butstillexpresstheearlymarkerPeroxidasin.ThesePeroxidasin-expressinghemocytesfailtomigratetotheirnormallocationsanddonotcompletetheirconversionintomacrophages.Ourresultssuggestthatbothgcmandgcm2arerequiredtogetherfortheproliferationofplasmatocyteprecursors,theexpressionofCroquemortprotein,andtheabilityofplasmatocytestoconvertintomacrophages.

【 授权许可】

Unknown