Developmental Biology | |
Peripheral Glia Direct Axon Guidance across the CNS/PNS Transition Zone | |
Joost Schulte1  Katharine J. Sepp1  Vanessa J. Auld1  | |
[1] Department of Zoology, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada | |
关键词: glia; axon; migration; pathfinding; Drosophila; grim; motor; sensory; ablation; gcm; peripheral; | |
DOI : 10.1006/dbio.2001.0411 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
CNSgliahaveintegralrolesindirectingaxonmigrationofbothvertebratesandinsects.Incontrast,verylittleisknownabouttherolesofPNSgliainaxonalpathfinding.InvertebratesandDrosophila,anatomicalevidenceshowsthatperipheralgliaprefigurethetransitionzonesthroughwhichaxonsmigrateintoandoutoftheCNS.Therefore,peripheralgliacouldguideaxonsatthetransitionzone.WeusedtheDrosophilamodelsystemtotestthishypothesisbyablatingperipheralgliaearlyinembryonicneurodevelopmentviatargetedoverexpressionofcelldeathgenesgrimandced-3.Theeffectsofperipheralgliallossonsensoryandmotorneurondevelopmentwereanalyzed.MotoraxonsinitiallyexittheCNSinabnormalpatternsintheabsenceofperipheralglia.However,theymustuseothercueswithintheperipherytofindtheircorrecttargetmusclessinceearlypathfindingerrorsarelargelyovercome.Whenperipheralgliaarelost,sensoryaxonsshowdisruptedmigrationastheytravelcentrally.Thisisnotaresultofmotorneurondefects,asdeterminedbymotor/sensorydouble-labelingexperiments.WeconcludethatperipheralgliaprefiguretheCNS/PNStransitionzoneandguideaxonsastheytraversethisregion.
【 授权许可】
Unknown
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