Peripheral Glia Direct Axon Guidance across the CNS/PNS Transition Zone

Developmental Biology

Joost Schulte¹  Katharine J. Sepp¹  Vanessa J. Auld¹ 

[1] Department of Zoology, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

关键词: glia;    axon;    migration;    pathfinding;    Drosophila;    grim;    motor;    sensory;    ablation;    gcm;    peripheral;   

DOI  :  10.1006/dbio.2001.0411

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

CNSgliahaveintegralrolesindirectingaxonmigrationofbothvertebratesandinsects.Incontrast,verylittleisknownabouttherolesofPNSgliainaxonalpathfinding.InvertebratesandDrosophila,anatomicalevidenceshowsthatperipheralgliaprefigurethetransitionzonesthroughwhichaxonsmigrateintoandoutoftheCNS.Therefore,peripheralgliacouldguideaxonsatthetransitionzone.WeusedtheDrosophilamodelsystemtotestthishypothesisbyablatingperipheralgliaearlyinembryonicneurodevelopmentviatargetedoverexpressionofcelldeathgenesgrimandced-3.Theeffectsofperipheralgliallossonsensoryandmotorneurondevelopmentwereanalyzed.MotoraxonsinitiallyexittheCNSinabnormalpatternsintheabsenceofperipheralglia.However,theymustuseothercueswithintheperipherytofindtheircorrecttargetmusclessinceearlypathfindingerrorsarelargelyovercome.Whenperipheralgliaarelost,sensoryaxonsshowdisruptedmigrationastheytravelcentrally.Thisisnotaresultofmotorneurondefects,asdeterminedbymotor/sensorydouble-labelingexperiments.WeconcludethatperipheralgliaprefiguretheCNS/PNStransitionzoneandguideaxonsastheytraversethisregion.

【 授权许可】

Unknown