| Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society | |
| Cofilin regulator 14‐3‐3ζ is an evolutionarily conserved protein required for phagocytosis and microbial resistance | |
| 关键词: Drosophila; zebrafish; plasmatocyte; macrophage; immunity; | |
| DOI : 10.1189/jlb.0410195 | |
| 学科分类：生理学 | |
| 来源: Federation of American Societies for Experimental Biology | |
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【 摘 要 】
Phagocytosisisanancientcellularprocessthatplaysanimportantroleinhostdefense.InDrosophilamelanogasterphagocytic,macrophage‐likehemocytesrecognizeandingestmicrobes.WeperformedanRNAi‐basedinvitroscreenintheDrosophilahemocytecelllineS2andidentifiedAbi,cpa,cofilinregulator14‐3‐3ζ,tlk,CG2765,andCG15609asmediatorsofbacterialphagocytosis.Oftheseidentifiedgenes,14‐3‐3ζhadanevolutionarilyconservedroleinphagocytosis:bacterialphagocytosiswascompromisedwhen14‐3‐3ζwastargetedwithRNAiinprimaryDrosophilahemocytesandwhentheorthologousgenesYwhabandYwhazweresilencedinzebrafishandmouseRAW264.7cells,respectively.InDrosophilaandzebrafishinfectionmodels,14‐3‐3ζwasrequiredforresistanceagainstStaphylococcusaureus.Weconcludethat14‐3‐3ζisessentialforphagocytosisandmicrobialresistanceininsectsandvertebrates...
【 授权许可】
CC BY
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201901233560021ZK.pdf | 2641KB |  download |
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