Adenosine deaminase potentiates the generation of effector, memory, and regulatory CD4+ T cells

Journal of Leukocyte Biology

José M. Gatell–¹  Carme Lluis and⁴  Felipe Garcia–¹  Núria Climent–²  Rodrigo Pacheco³  Isaac Naval-Macabuhay⁴  Josefa Mallol⁴  Víctor Casanova⁴  Rafael Franco⁴  José M. Martinez-Navio⁴  Teresa Gallart–² 

[1]–AIDS Unit, Hospital Clínic, Institut d²Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), and HIVACAT, Hospital Clínic, Faculty of Medicine, University of Barcelona, Spain

[2]

[3]Services of Immunology and Infectious Diseases & –AIDS Unit, Hospital Clínic, Institut d²Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS),

[4] Fundación Ciencia para la Vida and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile

[5] and Universidad San SebastiÃn, Chile

[6]Department of Biochemistry and Molecular Biology, Faculty of Biology, Services of Immunology and Infectious Diseases &

关键词: dendritic cells;    CD26;    T cell activation;    Th1;    CD45RA/CD45RO;    Foxp3;   

DOI  :  10.1189/jlb.1009696

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

By interacting with CD26 on the CD4+ T cell surface and with the AdoR A2B on the DC surface, ADA triggers a costimulatory signal for human T cells. The aim of this study was to know whether ADA-mediated costimulation plays a role in the differentiation of T cells. The results show that irrespective of its enzymatic activity and dependent on TNF-α, IFN-γ, and IL-6 action, ADA enhanced the differentiation of CD4+CD45RA+CD45RO– naïve T cells toward CD4+CD25+CD45RO+ Teffs and CD4+CD45RA–CD45RO+ memory T cells. Furthermore, ADA potentiated generation of CD4+CD25highFoxp3+ Tregs by a mechanism that seems to be mainly dependent on the enzymatic activity of ADA. Interestingly, an ADA-mediated increase on Teff, memory T cell, and Treg generation occurred, not only in cocultures from healthy individuals but also from HIV-infected patients. These data suggest that ADA is a relevant modulator of CD4+ T cell differentiation, even in cells from immunologically compromised individuals.

【 授权许可】

Unknown