Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage

Journal of Leukocyte Biology

Jeffrey D. Lifson⁶  Elkan F. Halpern²  Michael S. McGrath¹  Kenneth Williams⁵  Yue Sun⁵  Woong-Ki Kim⁵  Tricia H. Burdo⁴  Michael Piatak Jr.⁶  Patrick Autissier⁵  Hien Do³ 

[1] Pathologica, LLC, San Francisco, California, USA; Department of Laboratory Medicine, Positive Health Program, University of California, San Francisco, California, USA Pathologica, LLC, San Francisco, California, USA; Pathologica, LLC, San Francisco, California, USA; Department of Laboratory Medicine, Positive Health Program, University of California, San Francisco, California, USA Department of Laboratory Medicine, Positive Health Program, University of California, San Francisco, California, USA Pathologica, LLC, San Francisco, California, USA; Department of Laboratory Medicine, Positive Health Program, University of California, San Francisco, California, USADepartment of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA;Pathologica, LLC, San Francisco, California, USA; Pathologica, LLC, San Francisco, California, USA; Pathologica, LLC, San Francisco, California, USA;;Department of Biology, Boston College, Chestnut Hill, Massachusetts, USA; and Department of Biology, Boston College, Chestnut Hill, Massachusetts, USA; and Department of Biology, Boston College, Chestnut Hill, Massachusetts, USA; and;Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, and Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, and Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, andAIDS Vaccine Program, SAIC Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland, USA; AIDS Vaccine Program, SAIC Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland, USA; AIDS Vaccine Program, SAIC Frederick, Inc., National Cancer Institute at Frederick, Frederick, Maryland, USA;

关键词: subsets;    macrophage;    CD16;    HIV;    AIDS;   

DOI  :  10.1189/jlb.0209082

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Infection by HIV is associated with the expansion of monocytes expressing CD16 antigens, but the significance of this in HIV pathogenesis is largely unknown. In rhesus macaques, at least three subpopulations of blood monocytes were identified based on their expression of CD14 and CD16: CD14highCD16−, CD14highCD16low, and CD14lowCD16high. The phenotypes and functions of these subpopulations, including CD16+ monocytes, were investigated in normal, uninfected rhesus macaques and macaques that were infected with SIV or chimeric SHIV. To assess whether these different monocyte subpopulations expand or contract in AIDS pathogenesis, we conducted a cross-sectional study of 54 SIV- or SHIV-infected macaques and 48 uninfected controls. The absolute numbers of monocyte populations were examined in acutely infected animals, chronically infected animals with no detectable plasma virus RNA, chronically infected animals with detectable plasma virus RNA, and animals that died with AIDS. The absolute numbers of CD14highCD16low and CD14lowCD16high monocytes were elevated significantly in acutely infected animals and chronically infected animals with detectable plasma virus RNA compared with uninfected controls. Moreover, a significant, positive correlation was evident between the number of CD14highCD16low or CD14lowCD16high monocytes and plasma viral load in the infected cohort. These data show the dynamic changes of blood monocytes, most notably, CD14highCD16low monocytes during lentiviral infection, which are specific to disease stage.

【 授权许可】

Unknown