Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: subsets;    macrophage;    CD16;    HIV;    AIDS;   

DOI  :  10.1189/jlb.0209082

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

InfectionbyHIVisassociatedwiththeexpansionofmonocytesexpressingCD16antigens,butthesignificanceofthisinHIVpathogenesisislargelyunknown.Inrhesusmacaques,atleastthreesubpopulationsofbloodmonocyteswereidentifiedbasedontheirexpressionofCD14andCD16:CD14highCD16−,CD14highCD16low,andCD14lowCD16high.Thephenotypesandfunctionsofthesesubpopulations,includingCD16+monocytes,wereinvestigatedinnormal,uninfectedrhesusmacaquesandmacaquesthatwereinfectedwithSIVorchimericSHIV.ToassesswhetherthesedifferentmonocytesubpopulationsexpandorcontractinAIDSpathogenesis,weconductedacross‐sectionalstudyof54SIV‐orSHIV‐infectedmacaquesand48uninfectedcontrols.Theabsolutenumbersofmonocytepopulationswereexaminedinacutelyinfectedanimals,chronicallyinfectedanimalswithnodetectableplasmavirusRNA,chronicallyinfectedanimalswithdetectableplasmavirusRNA,andanimalsthatdiedwithAIDS.TheabsolutenumbersofCD14highCD16lowandCD14lowCD16highmonocyteswereelevatedsignificantlyinacutelyinfectedanimalsandchronicallyinfectedanimalswithdetectableplasmavirusRNAcomparedwithuninfectedcontrols.Moreover,asignificant,positivecorrelationwasevidentbetweenthenumberofCD14highCD16loworCD14lowCD16highmonocytesandplasmaviralloadintheinfectedcohort.Thesedatashowthedynamicchangesofbloodmonocytes,mostnotably,CD14highCD16lowmonocytesduringlentiviralinfection,whicharespecifictodiseasestage...

【 授权许可】

CC BY