Human circulating CD14+ monocytes as a source of progenitors that exhibit mesenchymal cell differentiation

Journal of Leukocyte Biology

Masataka Kuwana²  Yasuo Ikeda⁴  Yoko Ogawa³  Hidekata Yasuoka¹  Yutaka Kawakami²  Keisuke Izumi²  Yuka Okazaki²  Hiroaki Kodama⁴ 

[1] Institute for Advanced Medical Research; Department of Internal Medicine; and Institute for Advanced Medical Research; Institute for Advanced Medical Research; Department of Internal Medicine; and Department of Internal Medicine; and Institute for Advanced Medical Research; Department of Internal Medicine; andInstitute for Advanced Medical Research; Institute for Advanced Medical Research; Institute for Advanced Medical Research;;Institute for Advanced Medical Research; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan Institute for Advanced Medical Research; Institute for Advanced Medical Research; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan Institute for Advanced Medical Research; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan;Department of Internal Medicine; and Department of Internal Medicine; and Department of Internal Medicine; and

关键词: fibronectin;    lineage;    mesenchymal stem cell;    plasticity;   

DOI  :  10.1189/jlb.0403170

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

Circulating CD14+ monocytes are precursors of phagocytes, such as macrophages and dendritic cells. Here we report primitive cells with a fibroblast-like morphology derived from human peripheral blood CD14+ monocytes that can differentiate into several distinct mesenchymal cell lineages. We named this cell population monocyte-derived mesenchymal progenitor (MOMP). MOMPs were obtained in vitro from human peripheral blood mononuclear cells cultured on fibronectin in the presence of fetal bovine serum alone as a source of growth factors. MOMPs had a unique molecular phenotype–CD14+CD45+CD34+type I collagen+–and showed mixed morphologic and molecular features of monocytes and endothelial and mesenchymal cells. MOMPs were found to be derived from a subset of circulating CD14+ monocytes, and their differentiation required that they bind fibronectin and be exposed to one or more soluble factors derived from peripheral blood CD14− cells. MOMPs could be expanded in culture without losing their original phenotype for up to five passages. The induction of MOMPs to differentiate along multiple limb-bud mesodermal lineages resulted in the expression of genes and proteins specific for osteoblasts, skeletal myoblasts, chondrocytes, and adipocytes. Our findings represent the first evidence that human circulating CD14+ monocytes are a source of progenitors that exhibit mesenchymal cell differentiation.

【 授权许可】

Unknown