Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama

Memórias do Instituto Oswaldo Cruz

Octavio E Sousa²  Franklyn Samudio¹  Corina De Juncá²  José E Calzada¹ 

[1] ,University of Panama Faculty of Medicine Center for Research and Diagnosis of Parasitic Diseases,Panama

关键词: Trypanosoma cruzi;    lineage;    acute Chagas disease;    Panama;   

DOI  :  10.1590/S0074-02762006000400018

来源: SciELO

【 摘 要 】

The present work provides information on Trypanosoma cruzi genotype circulating in endemic areas of Chagas disease in Panama. A total of 26 crude stocks of T. cruzi, isolated from the blood of persons with different clinical profiles of Chagas disease were collected and crio-conserved until used. Most of the stocks had been characterized by means of isoenzyme electrophoresis on cellulose acetate membranes. The clinical profiles of infected persons included 9 (34.6%) asymptomatic and 17 acute (65.4%) including 5 (19.2%) fatal cases, 2 under 5 years old and 3 adults. A multiplex-PCR assay based on the amplification of the non-transcribed spacer of the mini-exon gene was performed. All stocks of T. cruzi included in the study were found to correspond to Tc I group. This result supports the predominance of T. cruzi-I in the transmission cycles affecting the human population in the Republic of Panama.

【 授权许可】

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