Essential involvement of IL-6 in the skin wound-healing process as evidenced by delayed wound healing in IL-6-deficient mice

Journal of Leukocyte Biology

Tatsunori Takayasu¹  Yuko Ishida¹  Toshikazu Kondo¹  Naofumi Mukaida²  Zi-Qing Lin³ 

[1] Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and;Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Japan Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Japan Division of Molecular Bioregulation, Cancer Research Institute, Kanazawa University, Japan;Faculty of Forensic Medicine, China Criminal Police College, Shenyang, China; and Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and Faculty of Forensic Medicine, China Criminal Police College, Shenyang, China; and Faculty of Forensic Medicine, China Criminal Police College, Shenyang, China; and Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and Faculty of Forensic Medicine, China Criminal Police College, Shenyang, China; and Department of Forensic & Social Environmental Medicine, Graduate School of Medical Science, and

关键词: leukocyte recruitment;    angiogenesis;    collagen production;    chemokines;    TGF-β1;    VEGF;   

DOI  :  10.1189/jlb.0802397

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

To clarify interleukin (IL)-6 roles in wound healing, we prepared skin excisions in wild-type (WT) and IL-6-deficient BALB/c [knockout (KO)] mice. In WT mice, the wound area was reduced to 50% of original size at 6 days after injury. Microscopically, leukocyte infiltration was evident at wound sites. Furthermore, the re-epithelialization rate was ∼80% at 6 days after injury with increases in angiogenesis and hydroxyproline contents. The gene expression of IL-1, chemokines, adhesion molecules, transforming growth factor-β1, and vascular endothelial growth factor was enhanced at the wound sites. In contrast, the enhanced expression of these genes was significantly reduced in KO mice. Moreover, in KO mice, the reduction of wound area was delayed with attenuated leukocyte infiltration, re-epithelialization, angiogenesis, and collagen accumulation. Finally, the administration of a neutralizing anti-IL-6 monoclonal antibody significantly delayed wound closure in WT mice. These observations suggest that IL-6 has crucial roles in wound healing, probably by regulating leukocyte infiltration, angiogenesis, and collagen accumulation.

【 授权许可】

Unknown