The performance of four molecular methods for the laboratory diagnosis of congenital toxoplasmosis in amniotic fluid samples

Revista da Sociedade Brasileira de Medicina Tropical

Kanunfre, Kelly Aparecida¹  Universidade de Sao Paulo, Sao Paulo, Brazil¹  Targa, Lilia Spaleta¹  Teixeira, Leandro Emidio¹  Yamamoto, Lidia¹  Rodrigues, Jonatas Cristian¹  Shimokawa, Paulo Tadashi¹  Okay, Thelma Suely¹  Domingues, Wilson¹ 

关键词: Congenital toxoplasmosis;    Congenital infection;    Molecular diagnosis;    PCR;    Quantitative PCR;    Real-time PCR;   

DOI  :  10.1590/0037-8682-0095-2013

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Introduction Toxoplasmosis may be life-threatening in fetuses and in immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence of IgM and IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim of this study was to compare the performance of 4 PCR-based methods for the laboratory diagnosis of toxoplasmosis. One hundred pregnant women who seroconverted during pregnancy were included in the study. The definition of cases was based on a 12-month follow-up of the infants.

【 授权许可】

Unknown