Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Ferreira, Marcelo Urbano¹  Pereira, Fabrício José Tarelho¹  Brienze, Vânia Maria Sabadoto¹  Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto¹  Santos, Manoel Armando Azevedo dos¹  Liso, Elisabete¹  Tonon, Ângela Pedroso¹  Tognola, Waldir Antônio¹  Universidade de São Paulo, São Paulo¹ 

关键词: Diagnosis of tuberculous meningitis;    PCR;    Mycobacterium tuberculosis;   

DOI  :  10.1590/S0037-86822001000400015

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

【 授权许可】

Unknown