Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Revista da Sociedade Brasileira de Medicina Tropical

Vânia Maria Sabadoto Brienze²  Ângela Pedroso Tonon²  Fabrício José Tarelho Pereira²  Elisabete Liso²  Waldir Antônio Tognola²  Manoel Armando Azevedo Dos Santos¹  Marcelo Urbano Ferreira¹ 

[1] ,Faculdade de Medicina de São José do Rio Preto Departamento de Doenças Infecciosas e Parasitárias São José do Rio Preto SP

关键词: Diagnosis of tuberculous meningitis;    PCR;    Mycobacterium tuberculosis;    Diagnóstico da meningite tuberculosa;    PCR;    Mycobacterium tuberculosis;   

DOI  :  10.1590/S0037-86822001000400015

来源: SciELO

【 摘 要 】

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

【 授权许可】

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