Nested-PCR using MPB64 fragment improves the diagnosis of pleural and meningeal tuberculosis

Revista da Sociedade Brasileira de Medicina Tropical

Costa, Fernando F.¹  Paschoal, Ilma A.¹  Silva, Silvana A.B.¹  Universidade de Campinas¹  Medical School, Marilia, Brazil¹  Universidade de Campinas, Campinas, Brazil¹  Martins, Luiz C.¹  Ward, Laura Sterian¹  Von Nowakonski, Angela¹ 

关键词: Tuberculosis;    Diagnosis;    PCR technique;    Central nervous system;    Pleura;   

DOI  :  10.1590/S0037-86822000000300003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

: Fluids in which Mycobacterium tuberculosis are seldom found, such as pleural and cerebrospinal liquids, are good candidates to be studied using PCR techniques. We detail our experience with a PCR assay applied to pleural and cerebrospinal fluids using the primer MPB64. Seventy three specimens were analyzed: 30 pleural fluids (PF), 26 pleural biopsies (PB) and 17 cerebrospinal fluids (CSF). The gold standard for the diagnosis of tuberculous meningitis was the positive culture for M. tuberculosis in CSF. Tuberculous pleural effusion was diagnosed when cultures of PF and/or PB were positive for M. tuberculosis, or the PB histology showed granulomas. Our results, compared to the gold standards employed, showed a sensitivity of 70%, specificity of 88%, positive predictive value of 82% and negative predictive value of 80%. The high specificity of the MPB64 fragment while still retaining a good sensitivity makes it very well suited for pleural and cerebrospinal tuberculosis diagnosis.

【 授权许可】

Unknown