Halistanol sulfate A and rodriguesines A and B are antimicrobial and antibiofilm agents against the cariogenic bacterium Streptococcus mutans

Revista Brasileira de Farmacognosia

Kossuga, Miriam H.¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Universidade Estadual de Campinas, Piracicaba, Brazil¹  Lima, Bruna de A.¹  Gonçalves, Reginaldo B.¹  Université Laval, Québec, Canada¹  Universidade de São Paulo, São Carlos, Brazil¹  Berlinck, Roberto G.S.¹  Universidade de São Paulo, Piracicaba, Brazil¹  Lira, Simone P. de¹  Kamiya, Regianne U.¹  Universidade Federal de Alagoas, Maceió, Brazil¹ 

关键词: Biofilms;    Molecular oral microbiology;    Dental caries;    Streptococcus;    Marine natural products;   

DOI  :  10.1016/j.bjp.2014.11.002

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

In the present investigation we report the antibacterial activity of halistanol sulfate A isolated from the sponge Petromica ciocalyptoides, as well as of rodriguesines A and B isolated from the ascidian Didemnum sp., against the caries etiologic agent Streptococcus mutans. The transcription levels of S. mutans virulence genes gtfB, gtfC and gbpB, as well as of housekeeping genes groEL and 16S, were evaluated by sqRT-PCR analysis of S. mutans planktonic cells. There were no alterations in the expression levels of groEL and 16S after antimicrobial treatment with halistanol sulfate A and with rodriguesines A and B, but the expression of the genes gtfB, gtfC and gbpB was down-regulated. Halistanol sulfate A displayed the most potent antimicrobial effect against S. mutans, with inhibition of biofilm formation and reduction of biofilm-associated gene expression in planktonic cells. Halistanol sulfate A also inhibited the initial oral bacteria colonizers, such as Streptococcus sanguinis, but at much higher concentrations. The results obtained indicate that halistanol sulfate A may be considered a potential scaffold for drug development in Streptococcus mutans antibiofilm therapy, the main etiologic agent of human dental caries.

【 授权许可】

Unknown