Studies on the application of immobilization technique for the production of cyclosporin A by a local strain of Aspergillus terreus

The Journal of General and Applied Microbiology

Lotfy A. R. Sallam¹  Hassaan A. El-Minofi¹  Abdel-Hamid A. Hamdi¹  Abdel-Monem H. El-Refai¹  Ibrahim S. Abd-Elsalam¹ 

[1] Microbial and Natural Products Chemistry Department, National Research Centre

关键词: Aspergillus terreus;    cyclosporin A;    immobilization;    production;   

DOI  :  10.2323/jgam.51.143

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The formation of cyclosporin A (Cy A) by immobilized spores and mycelia of Aspergillus terreus was investigated. Different carriers were tested as immobilizing carriers, whereby Ca-alginate was selected for further experimentation. The role of alginate concentration, biomass weight, pH value of the cultivation medium, repeated utilization of the immobilized fungus as well as the supplementation of different amino acid precursors were studied. Best Cy A outputs were attained with Ca-alginate 3% (w/v), mycelial weight 15% (w/v), pH 4.5 and four repeated cycles. Similarly, the Cy A productivity was markedly accelerated in the presence of L-valine and L-valine and L-leucine mixture.

【 授权许可】

Unknown