Utilization of nitriles by yeasts isolated from a Brazilian gold mine

The Journal of General and Applied Microbiology

João C. T. Dias²  Valter R. Linardi²  Fernando Carazza¹  Carlos A. Rosa²  Rachel P. Rezende² 

[1] Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais;Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais

关键词: amidase;    nitrile hydratase;    nitriles;    yeasts;   

DOI  :  10.2323/jgam.45.185

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Yeast strains from the genera Candida, Debaryomyces, Aureobasidium, Geotrichum, Pichia, Rhodotorula, Tremella, Hanseniaspora, and Cryptococcus were isolated from samples of a gold mine from liquid extraction circuit. These strains were tested for their ability to utilize acetonitrile at 12 mM as the sole nitrogen source. The yeasts that grew using acetonitrile at 12 mM were tested in the presence of acetonitrile, isobutyronitrile, methacrylnitrile, and propionitrile at concentrations of 12, 24, 48, 97, and 120 mM. One strain was selected for each nitrile and the concentration of nitrile in which the best growth occurred. Cryptococcus sp. strain UFMG-Y28 had a better growth on 120 mM propionitrile and 97 mM acetonitrile, Rhodotorula glutinis strain UFMG-Y5 on 48 mM methacrylnitrile, and Cryptococcus flavus strain UFMG-Y61 on 120 mM isobutyronitrile. The utilization of different nitriles and amides by yeast strains involves hydrolysis in a two-step reaction mediated by both inducible and intracellular nitrile hydratase and amidase.

【 授权许可】

Unknown