期刊论文详细信息
| Revista de microbiologia | |
| A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases | |
| Silva, Amanda Ribeiro Martins da1 Angelini, Leticia Mara Lima2 | |
| [1] Universidade Estadual Paulista Julio de Mesquita Filho, Araraquara, Brazil;Universidade Estadual Paulista Julio de Mesquita Filho, Rio Claro, Brazil | |
| 关键词: nitrile hydratase; amidase; nitrilase; amidase inhibitor; high-throughput screening.; | |
| DOI : 10.1590/S1517-838246120130851 | |
| 学科分类：农业科学（综合） | |
| 来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology | |
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【 摘 要 】
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
【 授权许可】
CC BY-NC
【 预 览 】
| Files | Size | Format | View |
|---|---|---|---|
| RO201910250697630ZK.pdf | 842KB |  download |
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