Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276

Molecules

Ricardo Lievano¹  Herminia Inés Pérez¹  Norberto Manjarrez¹  Aida Solís¹ 

[1] ¹Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana Unidad Xochimilco, Calzada del Hueso No. 1100, Col. Villa Quietud, Delegación Coyoacán, C.P. 04960 México, D.F., Mexico ²Centro de Investigación en Biotecnología Ambiental, Instituto Politécnico Nacional, Carretera Estatal Santa Inés Tecuexcomac-Tepetitla Km 1.5, C.P. 90700, Tlaxcala, Mexico

关键词: Nocardia corallina;    deracemisation;    ibuprofen;    nitrile hydratase;    amidase;   

DOI  :  10.3390/molecules17033148

来源: mdpi

【 摘 要 】

A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but the reactions are not enantioselective and low ee values were obtained. However, (R)-ibuprofen was isolated with >99% ee by a deracemisation process catalysed by N. corallina B-276. This is the first report of this kind of catalysis with this microorganism.

【 授权许可】

CC BY   
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