Contrasting transcriptome response to thermal stress in two key zooplankton species, Calanus finmarchicus and C. glacialis

Marine Ecology Progress Series

Arvind Y. M. Sundaram¹  Penelope Lindeque¹  Irina Smolina¹  Spyros Kollias¹  Jorge M. O. Fernandes¹  Eva F. Møller¹  Galice Hoarau¹ 

关键词: RNA-seq;    Copepods;    Temperature stress response;    Heat shock protein;    Climate change;   

DOI  :  10.3354/meps11398

学科分类：海洋学与技术

来源: Inter-Research

【 摘 要 】

ABSTRACT: Climate change has already led to the range expansion of warm-water plankton assemblages in the northeast Atlantic and the corresponding range contraction of colder-water species. The temperate copepod Calanus finmarchicus is predicted to shift farther northward into polar waters traditionally dominated by the arctic copepod C. glacialis. To identify temperature-mediated changes in gene expression that may be critical for the thermal acclimation and resilience of the 2 Calanus spp., we conducted a whole transcriptome profiling using RNA-seq on an Ion Torrent platform. Transcriptome responses of C. finmarchicus and C. glacialis from Disko Bay, west Greenland, were investigated under realistic thermal stresses (at +5, +10 and +15°C) for 4 h and 6 d. C. finmarchicus showed a strong response to temperature and duration of stress, involving up-regulation of genes related to protein folding, transcription, translation and metabolism. In sharp contrast, C. glacialis displayed only low-magnitude changes in gene expression in response to temperature and duration of stress. Differences in the thermal responses of the 2 species, particularly the lack of thermal stress response in C. glacialis, are in line with laboratory and field observations and suggest a vulnerability of C. glacialis to climate change.

【 授权许可】

Unknown