Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1

Revista de Microbiologia

Universidade Federal Rural de Pernambuco¹  Maia, Maria de Mascena Diniz¹  Morais Jr., Marcos Antonio de¹  Universidade Federal de Pernambuco, Recife, Brasil¹  Melo, Eduardo Henrique Magalhães¹  Morais, Marcia Maria Camargo de¹  Lima Filho, José Luiz de¹ 

关键词: : Enzyme kinetics;    ester hydrolysis;    extracellular lipase;    Fusarium solani;   

DOI  :  10.1590/S0001-37141999000400003

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25ºC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30ºC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50ºC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.

【 授权许可】

Unknown