An Efficient Method for Callus Formation of Protoplasts from Peanut (Arachis hypogaea L.) Leaf Mesophyll

Japanese Journal of Crop Science

Nisyawati¹  Takeshi TANIGUCHI¹  Hiroshi MIYAKE¹ 

关键词: Callus formation;    カルス形成;    Mesophyll protoplast;    プロトプラスト培養;    Peanut;    葉肉プロトプラスト;    Protoplast culture;    ラッカセイ;   

DOI  :  10.1626/jcs.63.362

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0.15% Pectolyase Y-23 and 0.5% Macerozyme R-10 was found to be the best for protoplast isolation. The protoplasts were then cultured in liquid or in agarose-gel modified KM8P medium. Cultured protoplasts divided 2 days after culture. In solidified media, protoplasts divided continually and formed many colonies 1 month after culture. The best viability and colony formation was obtained in a culture medium solidified with 1.2% agarose.

【 授权许可】

Unknown