Regeneration via Somatic Embryogenesis and/or Organogenesis in Callus from Mature Seeds of Echinochloa oryzicola Vasing

Japanese Journal of Crop Science

Yoshio KIKUTA¹  Kaien FUJINO¹  Zheng-Jun XU¹ 

关键词: Barnyard grass;    カルス形成;    Callus formation;    再分化;    Organogenesis;    タイヌエビ;    Regeneration;    不定芽形成;    Somatic embryogenesis.;    不定胚形成.;   

DOI  :  10.1626/jcs.62.614

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

Callus was initiated from mature seeds without the rinds of barnyard grass, Echinochloa oryzicola Vasing, on a modified Murashige and Skoog (MS) medium containing 2.0∼20.0 mgl-1 2, 4-D. The rate of callus formation was greatly improved with the addition of 4 mgl-1 tryptophan. Upon transfer to MS medium containing 6.0 mgl-1 of 2, 4-D, the calli were sustained in subcultures. The plantlets were regenerated under light on MS medium involving various hormone combinations of 2, 4-D and cytokinins. In the regenerated plantlets 55% were normal plants and 45% abnormal plants including albinos. The processes of plantlet regeneration were observed after the plantlets were embedded in paraffin. The ratio of embryogenesis to organogenesis was found to be 25 : 75 and this ratio could not be effectively controlled by media using varying concentration of 2, 4-D, BA, kinetin, and zeatin.

【 授权许可】

Unknown