Journal of Pharmacological Sciences | |
Microminipig, a Non-rodent Experimental Animal Optimized for Life Science Research: Novel Atherosclerosis Model Induced by High Fat and Cholesterol Diet | |
Hiroaki Kawaguchi1  Makoto Fujiki3  Hiroki Yoshida8  Ryoichi Nagata2  Yasukatsu Izumi4  Naoki Miura6  Masahisa Horiuchi9  Kazuhiro Misumi3  Noriaki Miyoshi5  Akihide Tanimoto7  Hiroaki Miyajima2  Toru Takeuchi9  | |
[1] Department of Veterinary Experimental Animal Science, Faculty of Agriculture, Kagoshima University, Japan;Shin Nippon Biomedical Laboratories, Ltd., Japan;Department of Surgery, Faculty of Agriculture, Kagoshima University, Japan;Department of Pharmacology, Osaka City University Medical School, Japan;Department of Veterinary Pathology, Faculty of Agriculture, Kagoshima University, Japan;Veterinary Teaching Hospital, Faculty of Agriculture, Kagoshima University, Japan;Department of Molecular and Cellular Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan;President, Kagoshima University, Japan;Department of Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Japan | |
关键词: atherosclerosis; cholesterol; diet; life-style; Microminipig; | |
DOI : 10.1254/jphs.10R17FM | |
学科分类:药学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
【 摘 要 】
References(35)Cited-By(20)Atherosclerotic lesions were observed in male and ovariectomized female Microminipig (MMP) fed a high fat and cholesterol diet with sodium cholate (HFCD/SC) for 3 months. HFCD/SC induced hypercholesterolemia accompanied by an increase in serum total cholesterol (T-Cho), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and cholesterol ester (CE). Unlike the mouse or rabbit, a dominant LDL-C fraction in the intact MMP, similar to that in humans, was observed by serum lipoprotein analysis. HFCD/SC increased body weight gain. At the end of the experiment, computed tomography scans of conscious animals showed that HFCD/SC had decreased liver attenuation values (Hounsfield unit) and increased subcutaneous and abdominal fat, suggesting the induction of fatty liver and obesity. HFCD/SC induced atherosclerotic lesions in systemic arteries, including the external and internal iliac arteries, abdominal aorta, coronary artery, and cerebral arterial circle. Atherosclerosis and pathological findings induced by HFCD/SC in MMP were similar to those in humans. The MMP is a potentially suitable tool for investigating human atherosclerosis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201911300998326ZK.pdf | 1138KB | download |