Microminipig, a Non-rodent Experimental Animal Optimized for Life Science Research: Novel Atherosclerosis Model Induced by High Fat and Cholesterol Diet

Journal of Pharmacological Sciences

Hiroaki Kawaguchi¹  Makoto Fujiki³  Hiroki Yoshida⁸  Ryoichi Nagata²  Yasukatsu Izumi⁴  Naoki Miura⁶  Masahisa Horiuchi⁹  Kazuhiro Misumi³  Noriaki Miyoshi⁵  Akihide Tanimoto⁷  Hiroaki Miyajima²  Toru Takeuchi⁹ 

[1] Department of Veterinary Experimental Animal Science, Faculty of Agriculture, Kagoshima University, Japan;Shin Nippon Biomedical Laboratories, Ltd., Japan;Department of Surgery, Faculty of Agriculture, Kagoshima University, Japan;Department of Pharmacology, Osaka City University Medical School, Japan;Department of Veterinary Pathology, Faculty of Agriculture, Kagoshima University, Japan;Veterinary Teaching Hospital, Faculty of Agriculture, Kagoshima University, Japan;Department of Molecular and Cellular Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Japan;President, Kagoshima University, Japan;Department of Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Japan

关键词: atherosclerosis;    cholesterol;    diet;    life-style;    Microminipig;   

DOI  :  10.1254/jphs.10R17FM

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(35)Cited-By(20)Atherosclerotic lesions were observed in male and ovariectomized female Microminipig (MMP) fed a high fat and cholesterol diet with sodium cholate (HFCD/SC) for 3 months. HFCD/SC induced hypercholesterolemia accompanied by an increase in serum total cholesterol (T-Cho), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and cholesterol ester (CE). Unlike the mouse or rabbit, a dominant LDL-C fraction in the intact MMP, similar to that in humans, was observed by serum lipoprotein analysis. HFCD/SC increased body weight gain. At the end of the experiment, computed tomography scans of conscious animals showed that HFCD/SC had decreased liver attenuation values (Hounsfield unit) and increased subcutaneous and abdominal fat, suggesting the induction of fatty liver and obesity. HFCD/SC induced atherosclerotic lesions in systemic arteries, including the external and internal iliac arteries, abdominal aorta, coronary artery, and cerebral arterial circle. Atherosclerosis and pathological findings induced by HFCD/SC in MMP were similar to those in humans. The MMP is a potentially suitable tool for investigating human atherosclerosis.

【 授权许可】

Unknown