Journal of Veterinary Medical Science | |
AP-1-Mediated Expression of Brain-Specific Class IVa β-Tubulin in P19Embryonal Carcinoma Cells | |
Emi KINOSHITA1  Kazuhiko ARAHARA1  Yuka MARUYAMA1  Katsuhiko ARAI1  | |
[1] Department of Tissue Physiology, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan | |
关键词: embryonal carcinoma cell; neuronal differentiation; promoter analysis; retinoic acid; tubulin isotype; | |
DOI : 10.1292/jvms.14-0343 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(36)Cited-By(1)Supplementary materials(1)Expression of brain-specificphenotypes increased in all trans retinoic acid (ATRA)-induced neuraldifferentiation of mouse P19 embryonal carcinoma cells. Among these phenotypes, expressionof class IVa β-tubulin isotype (TUBB4a) was particularly enhanced in neuraldifferentiation. Transient transfection assays employing a reporter construct found thatATRA-mediated regulatory region of the TUBB4a gene lay in the region from �?83 nt to +137nt relative to the +1 transcription start site. Site-directed mutagenesis in the AP-1binding site at �?29/�?17 suggested that the AP-1 binding site was a critical region forATRA-mediated TUBB4a expression. Chromatin immunoprecipitation experiments suggestedparticipation of JunD and activating transcription factor-2 (ATF2) in TUBB4a expression.Additionally, exogenous induction of the dominant-negative (dn) type of JunD canceledATRA-induced upregulation of TUBB4a, and the dn type of ATF2 suppressed even the basalactivity. Further immunoblot study revealed an ATRA-mediated increase in JunD protein,while a significant amount of ATF2 protein was constantly produced. These results suggestthat differentiation-mediated activation of JunD results in enhanced TUBB4aexpression.
【 授权许可】
Unknown
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