Role of miR-124 in the regulation of retinoic acid-induced Neuro-2A cell differentiation

Neural Regeneration Research

关键词: immunofluorescence;    map2;    microrna;    mir-124;    neuro-2a cells;    neurite outgrowth;    neuronal differentiation;    overexpression;    real-time pcr;    retinoic acid;   

DOI  :  10.4103/1673-5374.270417

来源: DOAJ

【 摘 要 】

Retinoic acid can cause many types of cells, including mouse neuroblastoma Neuro-2A cells, to differentiate into neurons. However, it is still unknown whether microRNAs (miRNAs) play a role in this neuronal differentiation. To address this issue, real-time polymerase chain reaction assays were used to detect the expression of several differentiation-related miRNAs during the differentiation of retinoic acid-treated Neuro-2A cells. The results revealed that miR-124 and miR-9 were upregulated, while miR-125b was downregulated in retinoic acid-treated Neuro-2A cells. To identify the miRNA that may play a key role, miR-124 expression was regulated by transfection of miRNA mimics or inhibitors. Morphological analysis results showed that inhibition of miR-124 expression reversed the effects of retinoic acid on neurite outgrowth. Moreover, miR-124 overexpression alone caused Neuro-2A cells to differentiate into neurons, and its inhibitor could block this effect. These results suggest that miR-124 plays an important role in retinoic acid-induced differentiation of Neuro-2A cells.

【 授权许可】

Unknown