Identification and Characterization of Lactic Acid Bacteria in a CommercialProbiotic Culture

Bioscience of Microbiota, Food and Health

Juan D. LATORRE¹  Neil R. PUMFORD¹  Marion J. MORGAN¹  Guillermo TELLEZ¹  Gopala KALLAPURA¹  Billy M. HARGIS¹  Anita MENCONI¹ 

[1] Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA

关键词: lactic acid bacteria;    probiotic;    identification;    characterization;    poultry;   

DOI  :  10.12938/bmfh.33.25

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

References(33)Cited-By(3)The aim of the present study was to describe the identification and characterization(physiological properties) of two strains of lactic acid bacteria (LAB 18 and 48) presentin a commercial probiotic culture, FloraMax®-B11. Isolates were characterizedmorphologically, and identified biochemically. In addition, the MIDI System ID, the BiologID System, and 16S rRNA sequence analyses for identification of LAB 18 and LAB 48 strainswere used to compare the identification results. Tolerance and resistance to acidic pH,high osmotic concentration of NaCl, and bile salts were tested in broth medium. Invitro assessment of antimicrobial activity against enteropathogenic bacteriaand susceptibility to antibiotics were also tested. The results obtained in this studyshowed tolerance of LAB 18 and LAB 48 to pH 3.0, 6.5% NaCl and a high bile saltconcentration (0.6%). Both strains evaluated showed in vitroantibacterial activity against Salmonella enterica serovar Enteritidis,Escherichia coli (O157:H7), and Campylobacter jejuni.These are important characteristics of lactic acid bacteria that should be evaluated whenselecting strains to be used as probiotics. Antimicrobial activity of these effectiveisolates may contribute to efficacy, possibly by direct antimicrobial activity invivo.

【 授权许可】

Unknown