Up-regulation of Muscle Uncoupling Protein 3 Gene Expression by Calcium Channel Blocker, Benidipine Hydrochloride in Rats

Endocrine Journal

Chizuko HIOKI²  Teruo KAWADA¹  Naoki SAKANE³  Toshihide YOSHIDA²  Kazuhiko KOTANI³ 

[1] Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University;Department of Endocrinology, Diabetes and Metabolism, Graduate School of Medical Science, Kyoto Prefectural University of Medicine;Department of Preventive Medicine, Clinical Research Institute, Center for Endocrine and Metabolic Disease, National Hospital Organization Kyoto Medical Center

关键词: Obesity;    Benidipine;    Uncoupling protein;    Brown adipose tissue;    Skeletal muscle;   

DOI  :  10.1507/endocrj.K06-130

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(26)Cited-By(1)To examine whether benidipine hydrochloride, one of the calcium channel blockers, up-regulate uncoupling protein 3 (UCP3) expression in two skeletal muscles (gastrocnemius and soleus) in rats. Wistar rats were treated orally with benidipine hydrochloride at 4 mg/kg for 7 days. Blood pressure was measured after 4 days. At the end of experiments, the rats were weighed, and brown adipose tissue (BAT) and skeletal muscles (gastrocnemius and soleus muscles) were removed. The mRNA levels of uncoupling protein 1 (UCP1) and UCP3 were measured using the real-time quantitative reverse transcription-polymerase chain reaction method. Benidipine reduced body weight and also had a hypotensive effect. In rats treated with benidipine, UCP1 mRNA levels were significantly increased 1.4-fold in BAT, and UCP3 mRNA levels in BAT and gastrocnemius muscle were significantly increased 1.7 and 3.0-fold, respectively, compared with the control rats. There was no difference in UCP3 mRNA levels in soleus muscle between the two groups. We concluded that benidipine up-regulates not only UCP1 gene expression in BAT but also UCP3 gene expression in BAT and gastrocnemius muscle, which may contribute to thermogenesis in rats.

【 授权许可】

Unknown