Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)

Journal of Veterinary Medical Science

Kenichi MASUDA³  Hajime TSUJIMOTO⁴  Keitaro OHMORI¹  Shigehiro IWABUCHI⁵  Toshihiro TSUKUI⁵  Keigo KURATA²  Sadatoshi MAEDA⁶  Masahiro SAKAGUCHI⁷ 

[1] Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–8509, Japan;Institute of Tokyo Environmental Allergy, 2–2–4 Yushima, Bunkyo-ku, Tokyo 113–0034, Japan;Animal Allergy Clinical Laboratories, Inc., 5–4–30 Nishihashimoto, Midori-ku, Sagamihara Kanagawa 252–0131, Japan;Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan;Nippon Zenyaku Kogyo Co., Ltd., 1–1 Tairanoue Sasagawa, Asaka-machi, Koriyama, Fukushima 963–0196, Japan;Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu, 501–1193, Japan;Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252–5201, Japan

关键词: atopic dermatitis;    canine;    FcεRIα;    IgE;    mite;   

DOI  :  10.1292/jvms.10-0520

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(1)To detect allergen-specific IgE in dogs with allergic diseases, we developed a recombinant canine high affinity IgE receptor α chain (FcεRIα)-based IgE detection system. Using the recombinant protein of canine FcεRIα expressed by an Escherichia coli expression system, we could detect house dust mite (Dermatophagoides farinae) allergen-specific IgE in sera from dogs naturally and experimentally sensitized to this allergen with ELISA and western blotting. The IgE binding activity of recombinant canine FcεRIα on ELISA was impaired by heat treatment of these sera. The specificity of this recombinant canine FcεRIα-based IgE detection system was confirmed by inhibition assays with canine IgE. The recombinant canine FcεRIα-based IgE detection system established in this study offers an alternative tool to measure allergen-specific IgE in dogs.

【 授权许可】

Unknown