Determination of Antigenic Proteins of Housedust Mites in 90 Dogs Suffering from Atopic Dermatitis

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA¹  Takeshi SAWAZAKI²  Chiharu FUJIWARA²  Kohei YAMASHITA²  Yoshiki NAKAO²  Ryouji AZUMA³ 

[1] Department of Pathobiology, Nihon University School of Veterinary Medicine;Pharmaceutical Research Laboratory, Hitachi Chemical Co., Ltd.;Azuma Animal Hospital

关键词: allergen;    atopic dermatitis;    canine;    housedust mite;    IgE;   

DOI  :  10.1292/jvms.64.673

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(6)Housedust mites, Dermatophagoides pteronyssinus (D. pteronyssinus) and Dermatophagoides farinae (D. farinae), are the important causative agents of allergic diseases in human and animals. By using 165 dogs suffering from atopic dermatitis (AD), serum levels of immunogloblin E (IgE) antibody against 25 kinds of allergen including housedust mites were determined. Housedust mites were the most frequent allergen against which 90 of the 165 allergic dogs (54.5%) by IMMUNODOT assay. With the further analysis of immunoblotting assay in the 90 dogs sensitized with housedust mites, antigenic proteins of housedust mites recognized by IgE antibodies were with the apparent molecular masses of 15, 76, 90, 98, and 170-kD. Among them, the 15-kD protein that might be identical to Group 2 antigens (Der f2, Der p2) was prominently observed (52/90). This study indicates that about a half of dogs with AD were sensitized to housedust mites, suggesting that Group 2 antigens of housedust mites may be a major allergen in canine AD.

【 授权许可】

Unknown