Molecular Characterization of Salmonella enterica Serovar Typhimurium Isolated from Human, Food, and Animal Sources in Malaysia

Japanese Journal of Infectious Diseases

Haruo Watanabe³  Bjørn-Arne Lindstedt¹  Kwai Lin Thong²  Soo Tein Ngoi² 

[1] Division of Infectious Diseases Control, Norwegian Institute of Public Health;Institute of Biological Sciences, Faculty of Science, University of Malaya;National Institute of Infectious Diseases

关键词: antimicrobial resistance;    flagellar phase;    genotyping;    molecular characterization;    Salmonella Typhimurium;   

DOI  :  10.7883/yoken.66.180

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

References(37)Cited-By(6)Salmonella Typhimurium is an important nontyphoidal Salmonella serovar associated with foodborne diseases in many parts of the world. This organism is the major causative agent of nontyphoidal salmonellosis in Malaysia. We aimed to investigate the genetic profiles of the strains isolated from clinical, zoonotic, and dietary sources in Malaysia using multilocus variable number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). By focusing on the 5 common variable number tandem repeat (VNTR) loci, we found that PFGE (D = 0.99) was more discriminative than MLVA (D = 0.76). The low MLVA score might be because of a lack of VNTR loci STTR6 (81.0%) and STTR10pl (76.2%). Both subtyping methods suggested that our S. Typhimurium strains were largely endemic with limited genetic variation. Furthermore, we observed that biphasic S. Typhimurium strains were dominant (99%) and multidrug resistance was prevalent (50%) within our sample pool. The most frequently observed phenotypes were resistance to compound sulfonamides (49%), tetracycline (51%), and streptomycin (52%). In this study, we documented the genetic relationship, antimicrobial resistance characteristics, and flagellar-phase dominance among S. Typhimurium strains found in Malaysia.

【 授权许可】

Unknown