Molecular epidemiology and antimicrobial susceptibility of Enterococci recovered from Brazilian intensive care units

Brazilian Journal of Infectious Diseases

Ricardo Titze-de-almeida¹  Maurício Rollo Filho²  Celeste A. Nogueira²  Isabela P. Rodrigues¹  João Eudes Filho²  Rejane S. Do Nascimento²  Renato F. Ferreira Ii²  Lídia M. P. Moraes²  Hélène Boelens²  Alex Van Belkum²  Maria Sueli Soares Felipe² 

[1] ,University Hospital of Brasília Molecular Microbiology and Biotechnology Laboratory

关键词: Enterococci;    antimicrobial resistance;    genotyping;   

DOI  :  10.1590/S1413-86702004000300002

来源: SciELO

【 摘 要 】

We studied the antimicrobial resistance and the molecular epidemiology of 99 enterococcal surveillance isolates from two hospitals of Brasília, Brazil. Conventional biochemical tests were used to identify the enterococcal species and the disk diffusion method was used to determine their resistance profiles. Enterococcus faecalis (76%) and E. faecium (9%) were the most prevalent species. No enterococci showed the vanA or vanB vancomycin resistance phenotypes or genotypes. Only the intrinsically resistant species E. gallinarum (n=2) and E. casseliflavus (n=3) harbored the vancomycin-resistance genes vanC1 and vanC2/3, respectively. We found E. faecalis isolates with high-level resistance to gentamicin (22%) and streptomycin (8%) and both E. faecalis and E. faecium isolates with resistance to more than two antimicrobials (84% and 67%, respectively). Nine E. faecalis isolates (12%) were resistant to ampicillin; the minimal inhibitory concentration (MIC) values were 16µg/mL (n=6) and 32µg/mL (n=3). Among these ampicillin-resistant E. faecalis, seven were also resistant to gentamicin, ciprofloxacin, rifampin, penicillin, chloramphenicol, tetracycline and erythromycin. Pulsed-field gel electrophoresis classified those isolates in three different genotypes, suggesting dissemination of genetically related ampicillin-resistant E. faecalis strains among different patients.

【 授权许可】

CC BY-NC-ND   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License