Molecular Characterization of Tb, a New Approach for an Ancient Brucellaphage

International Journal of Molecular Sciences

Cai-Zhong Zhu²  Hong-Yan Xiong²  Jing Han³  Bu-Yun Cui¹  Dong-Ri Piao¹  Ya-Fei Li²  Hai Jiang¹  Qian Ren²  Xiang-Yu Ma²  Ya-Ming Chai²  Xia Huang²  Hong-Yan Zhao¹ 

[1] State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC / Liu zi No.5, Changping district, Beijing, 102206, China; E-Mails:;Department of Epidemiology, College of Preventive Medicine, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China; E-Mails:;Institute of Combine Injury, School of Preventive Medicine, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University / Gaotanyan road 30, Shapingba district, Chongqing 400038, China; E-Mail:

关键词: brucellaphage;    molecular characterization;    random amplified polymorphic DNA;   

DOI  :  10.3390/ijms10072999

来源: mdpi

【 摘 要 】

Tb (Tbilisi), the reference Brucellaphage strain, was classified as a member of the Podoviridae family with icosahedral capsids (57 ± 2 nm diameter) and short tails (32 ± 3 nm long). Brucellaphage DNA was double stranded and unmethylated; its molecular size was 34.5 kilobase pairs. Some sequences were found through RAPD analysis, TA cloning technology, and structural proteins were observed by using SDS-PAGE. Thus, the results have laid the foundation for the wider use of Brucellaphage’s basic mechanisms and practical applications.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.