Random amplified polymorphic DNA analysis of Thunbergia laurifolia Lindl. and its related species

Dynamic Chiropractic

关键词: Acanthaceae;    DNA fingerprint;    molecular characterization;    random amplified polymorphic DNA;    Thunbergia laurifolia.;   

DOI  :  10.5897/JMPR11.1002

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

ThunbergialaurifoliaLindl.is an important herb in Thai folk medicine that has been widely used as an antidote, anti-inflammatory and antipyretic. However, counterfeits ofT. laurifoliahave been commonly found in Thai herbal markets and have arisen based on their similarity in appearance and shared common names. Flowers of plants belonging toThunbergiaspp., includingT. laurifolia, ThunbergiagrandifloraandThunbergiaerecta, look similar to each other, which often cause confusion when identifying these species. Moreover, there is another group of plants with names that share a similar common Thai name, “Rang Chuet”, which includes aCurcumasp.,Rinoreasp.,Crotalariaspectabilis, andT. laurifolia. In this study, we developed a random amplified polymorphic DNA (RAPD) method to facilitate the rapid detection of these medicinal materials. Out of 80 screened primers, nine gave clear and reproducible RAPD patterns. Among 164 amplified bands, 70 bands were polymorphic in six species. The dendrogram was constructed using unweightedpair group method with arithmetic averages(UPGMA), and the genotypes were differentiated into three main groups. The RAPD profiles of six species showed low similarity indices (0.1235 to 0.3137), whereas the genetic relationship was associated with the botanical characterization. These results indicate that RAPD is a useful tool for differentiatingT. laurifoliafrom its related species.

【 授权许可】

Unknown