Immunization of cultured juvenile rockfish Sebastes schlegeli against Microcotyle sebastis (Monogenea)

Diseases of Aquatic Organisms

Soo Il Park¹  Yoon Jung Hwang¹  Jae Bum Cho¹  Ki Hong Kim¹ 

关键词: Rockfish;    Microcotyle sebastis;    Immunization;    Injection;    Immersion;   

DOI  :  10.3354/dao040029

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: To determine whether immunization with Microcotyle sebastis antigen could induce protection against the parasite's establishment, naive juvenile rockfish were immunized by injection or immersion with whole worm antigen of M.sebastis. The infestation intensities of immunized groups following a challenge (2 wk after boosting) with 5000 M. sebastis eyed-eggs were significantly lower than those of control groups, when determined 7 wk postinfection. The fish in thegroups boosted with M. sebastis antigen showed stronger protection than unboosted groups. The control group injected with FCA only showed a significantly smaller number of worms than the control group, which was immersed in PBS containing seawater.The results strongly suggest that both specific and nonspecific immune factors participate in the protection of rockfish against M. sebastis establishment.

【 授权许可】

Unknown