Fine Structure of Atrial Natriuretic Peptide(ANP)-Granules in the Atrial Cardiocytes in the Pig, Cattle and Horse

Journal of Veterinary Medical Science

Hiroharu MIFUNE¹  Yasutaka NODA¹  Syusaku SUZUKI¹  Shiro MOHRI¹  Koshi MOCHIZUKI¹ 

关键词: ANP-granule;    cardiocyte;    domestic animal;    immunohistochemistry;    morphometric analysis;   

DOI  :  10.1292/jvms.53.561

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(14)In the pig, cattle and horse, the right and left atria and ventricles were examined by immunohistochemistry, and the right atrial and auricular cardiocytes were studied by transmission electron microscopy. Moreover, ANP-granules in the cardiocytes were analyzed by ultrastructural morphometry. Immunohistochemically, the most intensely ANP-reacted cardiocytes were localized in the right auricle, particularly more prominent in the pig and cattle than in the horse. Ultrastructurally, ANP-granules were located principally in the perinuclear region associated with the Golgi apparatus and throughout the sarcoplasmic layers. They were classified into 2 types, A and B. A-granule had a conspicuous electron-dense core enveloped by a membrane. B-granule had a fibrillogranular core enveloped by an indistinct membrane. By TEM equipped with a goniometer stage, a part of the limiting membrane of B-granules became visible as the stage tilt progressed. By ultrastructural morphometry, the number of each type of granules was significantly greater in the pig and cattle than in the horse. In the pig and cattle, the total number of both types of granules in the auricular cardiocytes was significantly greater than that in the atrial ones. The diameter of each type of granules was significantly larger in the pig and cattle than in the horse. The diameter of A-granules was significantly larger than that of B-granules.

【 授权许可】

Unknown