ACTH-Induced Cortisol Secretion Is Mediated by cAMP and PKC in Various Adrenocortical Adenomas

Endocrine Journal

TOSHIROH SHIBATA¹  TOMOATSU MUNE¹  KOUJI YAMADA¹  TETSUYA SUWA¹  TATSUO ISHIZUKA¹  KAZUO KAJITA¹  ATSUSHI MIURA¹  KEIGO YASUDA¹  SATOMI ITAYA¹  HIROYUKI MORITA¹  HISASHI DAIDOH¹ 

[1] The Third Department of Internal Medicine, Gifu University School of Medicine

关键词: Cortisol secretion;    ACTH;    Phorbol ester;    PKC;    Adrenocortical adenoma;   

DOI  :  10.1507/endocrj.44.661

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(25)Cited-By(1)We examined the role of PKC in cortisol secretion from adrenocortical adenomas. Isolated cells were prepared from aldosterone producing adenoma (APA, n=5), APA complicated with preclinical Cushing's syndrome (APA+PC, n=1), PC (n=2), and cortisol producing adenoma (CPA, n=5). They were stimulated with 100nM ACTH, 1μM forskolin (FS), 1μM tetradecanoyl phorbol 13-acetate (TPA), and 100nM angiotensin II (AngII). ACTH was most potent to secret Cortisol. FS also stimulated cortisol secretion, but did less-potently. TPA and AngII also stimulated cortisol secretion significantly in cells from CPA. Furthermore, ACTH- and TPA-induced PKCα and β translocations from cytosol to membrane were observed in adenoma cells from APA+PC, PC, and CPA. In conclusion, it was suggested that ACTH-induced cortisol secretion may be mediated by both PKC and protein kinase A in adrenocortical adenomas, and that PKC-mediated signal transduction may be involved in ACTH-induced cortisol secretion in CPA.

【 授权许可】

Unknown