Sphingosine‐1‐phosphate stimulates cortisol secretion

FEBS Letters

Rábano, Miriam¹  Peña, Ana¹  Macarulla, José Marı́a¹  Gómez-Muñoz, Antonio¹  Trueba, Miguel¹  Marino, Aida¹  Brizuela, Leyre¹ 

[1] Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Spain

关键词: Sphingosine-1-phosphate;    Ceramide;    Cortisol secretion;    Phospholipase D;    Protein kinase C;    Adrenal gland;    BSA;    bovine serum albumin;    C2-ceramide;    N-acetylsphingosine;    DMEM;    Dulbecco's modified Eagle's medium;    FBS;    fetal bovine serum;    IP3;    inositol trisphosphate;    LPA;    lysophosphatidate;    PA;    phosphatidate;    PKC;    protein kinase C;    PLD;    phospholipase D;    PI-PLC;    phosphatidylinositol-dependent phospholipase C;    PMA;    4β-phorbol 12-myristate 13-acetate;    PTX;    pertussis toxin;    Sph-1-P;    sphingosine-1-phosphate;    ZF;    zona fasciculata;   

DOI  :  10.1016/S0014-5793(02)03882-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show here for the first time that sphingosine-1-phosphate (Sph-1-P) stimulates cortisol secretion in zona fasciculata cells of bovine adrenal glands. This effect was dependent upon protein kinase C (PKC) and extracellular Ca²⁺, and was inhibited by pertussis toxin. Sph-1-P activated phospholipase D (PLD) through a pertussis toxin-sensitive mechanism, also involving extracellular Ca²⁺ and PKC. Primary alcohols, which attenuate formation of phosphatidic acid (the product of PLD), and cell-permeable ceramides, which inhibit PLD, blocked Sph-1-P-induced cortisol secretion. In conclusion, Sph-1-P stimulates cortisol secretion through a mechanism involving Gi/o protein-coupled receptors, extracellular Ca²⁺, PKC and PLD.

【 授权许可】

Unknown