Both Antisense and Sense RNAs against the Nucleocapsid Protein Gene Inhibit the Multiplication of Mouse Hepatitis Virus

Journal of Veterinary Medical Science

Akihiko MAEDA¹  Masanobu HAYASHI¹  Shigeo NAMIOKA¹  Tetsuya MIZUTANI¹  Hiroshi ISOGAI¹ 

关键词: antisense RNA;    mouse hepatitis virus;    nucleocapsid protein;    sense RNA;   

DOI  :  10.1292/jvms.56.211

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Cited-By(1)DBT cells and several transfected cell lines which express antisense or sense RNA against the nucleocapsid protein gene of mouse hepatitis virus (MHV) were examined for the yields of MHV. The transfected cells showed 95 and 99% reduction of virus yield at 9 and 12 hr postinfection (p.i.) as compared with untransfected DBT cells. A remarkable decrease in MHV-specific RNA synthesis was observed in both transfected cell lines at 3.5 hr p.i. The result suggested that both antisense and sense RNAs inhibited viral replication at the initial stage of infection.

【 授权许可】

Unknown