Inhibition of Viral Multiplication in Cells Chronically Infected with Mouse Hepatitis Virus by Antisense RNA against the Polymerase Gene

Journal of Veterinary Medical Science

Akihiko MAEDA¹  Tomomasa WATANABE¹  Masanobu HAYASHI¹  Kozue ISHIDA¹  Shigeo NAMIOKA¹  Tetsuya MIZUTANI¹ 

关键词: antisense RNA;    chronic infection;    mouse hepatitis virus;   

DOI  :  10.1292/jvms.57.563

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(2)Recently, we showed that the antisense RNA containing a hammerhead ribozyme sequence against the polymerase gene of mouse hepatitis virus (MHV) inhibited viral multiplication in acute infection [10]. In the present study, we examined the inhibitory effects of an antisense RNA on viral multiplication in chronic MHV infection. In cell line LR-2, in which the 926-nucleotide (nt) antisense RNA containing a ribozyme sequence against the polymerase gene was expressed constitutively at a high level, chronic MHV infection was established through the maintenance of infection over 100 days postinfection (d.p.i.). After 200 d.p.i., no infectious progeny virus was observed in the culture medium of chronically MHV infected LR-2 cells. Our present results showed that the antisense RNA could also inhibit viral multiplication in chronic MHV infection.

【 授权许可】

Unknown