Small Molecule Lactoferrin with an Inflammatory Effect But No Apparent Antibacterial Activity in Mastitic Mammary Gland Secretion

Journal of Veterinary Medical Science

Yumiko KOMINE³  Yoshiaki OBARA²  Katsuo KUMAGAI³  Jin KOBAYASHI¹  Toshinobu KUROISHI³  Ken-ichi KOMINE³ 

[1] Department of Animal Reproduction, Miyagi Agricultural College Research Farm;Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University;T-Cell Research Institute, In Kyouitsu Seiyaku Corp.

关键词: concanavalin A;    lactoferrin;    mammary gland secretion;    mastitis;   

DOI  :  10.1292/jvms.67.667

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(37)Cited-By(11)We have identified various lactoferrin (Lf) molecules in mastitic mammary gland secretions (MGSs), and these Lf molecules were examined for their physiological function in MG. These Lf molecules were isolated by Con A affinity chromatography, and then analyzed by various electrophoresis methods and N-terminal amino acid sequencing. The low Con A affinity Lf was found to have low molecular peptides as compared with the 86 kDa of the high Con A affinity Lf, which is usually detected in healthy MGSs. The N-terminal amino acid sequence of each of the small molecular Lfs were confirmed as fragments of 86 kDa Lf. This low Con A affinity Lf stimulated spleen adherent cells to produce more O2- than 86 kDa Lf. Furthermore, the low Con A affinity Lf showed low antibacterial activity against E. coli and S. aureus, and had decreased iron-binding capacity in comparison with 86 kDa Lf. Moreover, the 86 kDa Lf could stimulate bovine T cells or macrophages to produce IFN-γ, IL-4, IL-6, and IL-1α. However low Con A affinity Lf induced the production of TNFα, but not physiological T cell or macrophage cytokines. It was also found that when the healthy MGs of dry cows were injected with the low Con A affinity Lf, there was an increase in polymorphonuclear cells together with TNFα, MCP-1, and IL-8 production. These results suggested that low Con A affinity Lf in mastitic MGSs differed from 86 kDa Lf in physiological characteristics, and, that it induced an inflammatory reaction in MGs.

【 授权许可】

Unknown