Effect of Combination Therapy with Lactoferrin and Antibiotics against Staphylococcal Mastitis on Drying Cows

Journal of Veterinary Medical Science

Masashi ITAGAKI²  Yumiko KOMINE⁴  Yoshiaki OBARA³  Katsuo KUMAGAI⁴  Kenzou KAI⁴  Toshinobu KUROISHI⁴  Hisashi ASO¹  Ken-ichi KOMINE⁴ 

[1] Laboratory of Functional Morphology, Division of Life Science, Graduate School of Agricultural Science, Tohoku University;Okitama Veterinary Clinical Center, Yamagata Prefectural Federation Agricultural Mutual Aid Association;Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University;T-Cell Research Institute, In Kyouritsu Seiyaku Co.

关键词: lactoferrin;    mastitis;    nuclear factor κ B;    Staphylococcus aureus;   

DOI  :  10.1292/jvms.68.205

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(42)Cited-By(7)We examined combination therapy with both lactoferrin (Lf) and antibiotics on clinical mastitis due to Staphylococcus aureus (S.aureus) on drying cows. The clinical symptoms of mastitic quarters were cured 81% of combination therapeutic quarters at 7 days post injection (dpi). Moreover, most of mammary gland secretions (MGSs) in combination therapeutic quarters were normal at 7 days after parturition. In the quarters with combination therapy, S.aureus counts, Lf concentrations and content rate of concanavalin A (Con A) low-affinity Lf decreased and were lower than in the quarters treated with Lf or antibiotics alone. The mRNA expression of tumor necrosis factor α (TNFα) of the quarters with combination therapy also decreased and was lower than that of the Lf or antibiotics treated. The mRNA expression of pro-inflammatory cytokines and chemokines in bovine mammary gland epithelial lined cells (BMEC) stimulated with Lf were lower than those of Con A low-affinity Lf stimulated BMEC. Moreover, Lf showed an inhibitory effect to the induction of pro-inflammatory cytokine mRNA expression when co-stimulated with Lf and Con A low-affinity Lf. Nuclear factor kappa B (NFκB) activation was also induced with Con A low-affinity Lf, and the inhibitory effects of Lf were also confirmed on BMEC co-stimulated with Lf and Con A low-affinity Lf. These results indicated that the efficacy of combination therapy with antibiotics and Lf caused antibacterial effect of antibiotics and inhibition of pro-inflammatory cytokine and chemokine production with Lf via the inhibition of NFκB activation.

【 授权许可】

Unknown